垂花蕙兰丛生芽途径的组织培养技术研究

摘要

Lateral buds were used as the explants for the study on tissue culture of pendent Cymbidium .It showed that by using 72% ethanol ,0.1% mercuric chloride and 15% sodium hypochlorite to disinfect the lateral buds three times ,the bud survival rate could be as high as 56% .The disinfection was especially effective in springtime .The induction rate of the clustered shoots was up to 100% with numerous differentiated buds when 6-BA was applied at the rate of 4.0 mg · L -1 .During the proliferating period of the clustered shoots ,the 6-BA treatment was a key factor that produced the best effect with a proliferating coefficient of clustered shoots of 3.4 and strong buds .For the development in the rooting stage ,on the other hand ,a 1/2 MS medium containing NAA 2.0 mg · L -1 + banana slurry 50 g · L -1 +AC 1.0 g · L -1 appeared to be most desirable .And ,moss seemed to be the best base material for the cultivation of the pendent Cymbidium plantlets .%以侧芽为外植体，对垂花蕙兰丛生芽途径的组织培养进行研究，结果表明，采用72％乙醇、0.1％升汞和15％次氯酸钠对侧芽进行3次消毒，存活率高达56％，尤其是在春季外植体消毒效果佳；在丛生芽诱导的过程中，6-BA浓度为4.0 mg · L -1时，丛芽诱导率可达100％且丛芽分化较多；在丛生芽增殖过程中，6-BA是影响垂花蕙兰丛生芽增殖的重要因素，6-BA 4.0 mg · L -1处理增殖系数为3.4，丛生芽较健壮，适合丛生芽增殖；培养基1/2MS＋NAA2.0 mg · L -1＋香蕉泥50 g · L -1＋AC1 g · L -1生根壮苗效果较好；苔藓是垂花蕙兰试管苗移栽较好的基质。