高效液相色谱法测定鳗鲡各组织中的乙酰甲喹残留量

摘要

An HPLC methodology was developed for determining mequindox residues in the organs of eels.The analysis was conducted under the following conditions:SunFireTM C18 column (4.6 mm×150 mm,3.5 μm)at 35℃,methanol:deionized water (v/v 40∶60)for themobile phase with a flow rate of 0.8 mL·min-1 ,and 239 nm wavelength seton PDA detector for optical density measurements.With in the mequindox concentration range of 0.05-2.5μg·mL-1 ,an excellent linear regression between the drug concentration and the peak area under curve was obtained with a correlation coefficient greater than 0.9 9 9.LODs of mequindox in the muscle,liver,kidney,and plasma from the eelswere 3 μg · kg-1 ,1 5μg · kg-1 ,1 5 μg · kg-1 ,and 1 5 μg · L-1 ,respectively.The average mequindox recovery rate of the method ranged from 76% to 90% with RSD (n=6)below 10%.It was,thus, concluded that the newly developed simple,rapidmethodology was highly sensitive and repeatable in detecting mequindox residuein the tissues of the eels.%建立鳗鲡各组织中乙酰甲喹残留量的高效液相色谱测定方法,高效液相色谱检测条件为：采用SunFireTM C18柱(4.6 mm×150 mm,3.5μm),柱温35℃；以甲醇∶水(40∶60,体积比)为流动相,流速为0.8 mL·min-1；采用二极管阵列检测器,检测波长239 nm。结果表明：在0.05~2.5μg·mL-1范围内,乙酰甲喹具有良好的线性关系,含量与峰面积的相关系数达0.999以上；乙酰甲喹在鳗鲡肌肉、肾、肝和血浆中的检出限分别为3μg·kg-1、15μg·kg-1、15μg·kg-1、15μg·L-1,鳗鲡各组织中乙酰甲喹回收率在76%~90%,相对标准偏差均小于10%(n=6)。本方法简便、快捷、灵敏度高,具有良好的重复性,满足鳗鲡各组织基质内乙酰甲喹残留量的快速定量分析的要求。