斑茅纤维降解生产燃料酒精的发酵工艺研究

摘要

Fermentation conditions for generating alcohol from cellulose hydrolytes of Saccharum arundinaceum were studied.The single factors applied for the experimentation included reaction time, temperature, pH, nitrogen source, yeast extract, and amount of yeast for inoculation.It indicated that a reacting time ranging from 72 to 96 h, temperature at 35 ℃, pH at 4.8-5.2, ammonium sulfate addition of 0.8%-1.0%, and yeast inoculation for 10%-15% could bring about a high efficiency for the alcohol generation.Subsequently, an orthogonal experiment optimized the fermentation conditions to be 35℃ for 84 h with an addition of 1.2% ammonium sulfate and 20% yeast that resulted in an alcohol yield of 32.62% at the concentration of 4.6% (V/V).%以能源植物斑茅纤维为原料,探讨纤维素糖化液生产燃料酒精的发酵工艺研究,分析了时间、温度、pH、氮源种类、酵母膏用量与酵母用量等因素对斑茅纤维素酒精发酵的影响.结果表明:反应时间72~96 h,温度35℃,pH4.8~5.2,酵母膏氮源用量0.8%~1.0%,酵母用量10%~15%时斑茅纤维糖化液的酒精发酵效果最好.通过正交试验确定了最佳发酵条件:时间84 h、温度35℃、酵母膏用量1.2%、酵母用量20%,发酵验证酒精值达4.6%(V/V),酒精得率32.62%.