大鼠肾系膜细胞饰胶蛋白聚糖乙酰化可增强其蛋白质稳定性和功能

摘要

Objective To detect the acetylation of decorin (DCN) and its influence on DCN ubiquitination in mesangial cells in rats.Methods Mesangial cells of rats were cultured in vitro.The immunoprecipitation,Western blot assay and RT-PCR were used to determine the acetylation of DCN.Results DCN was acetylated in renal mesangial cells in rats.The acetylated DCN promoted its stability via inhibiting of its degradation through polyubiquitination.Moreover,transforming growth factor-β1 (TGF-β1) and type Ⅳ collagen expression of mesangial cells decreased,and cell growth was inhibited when acetylation of DCN was enhanced in mesangial cells.Conclusions Acetylation of DCN inhibited DCN ubiquitination degradation,which enhances DCN's antagonistic effect against nephritis.These results may provide a potential target for further study of prevention and treatment of mesangial cell proliferative glomerulonephritis.%目的 通过细胞学研究检测大鼠肾系膜细胞饰胶蛋白聚糖(decorin,DCN)的乙酰化及其对系膜细胞泛素化的影响作用.方法 体外培养大鼠肾系膜细胞,应用免疫共沉淀、蛋白质电泳分析及RT-PCR等检测DCN的表达改变.结果 大鼠肾系膜细胞存在DCN乙酰化修饰,且DCN的乙酰化修饰抑制了DCN泛素化降解,促进了DCN蛋白质的稳定.此外增强DCN乙酰化修饰可促进转化生长因子β1(transforming growth factor-β1,TGF-β1)以及Ⅳ型胶原的下调,同时抑制肾系膜细胞的生长.结论 DCN乙酰化修饰可抑制DCN降解,增强DCN抗肾炎的作用,在防治系膜细胞增生性肾炎中可作为潜在的干扰靶点.