应用PCR技术扩增了中国主要养殖鲑亚科鱼类(5属10种)的线粒体控制区部分序列, 并研究这些鱼类的系统发育关系. 经序列比对后获得728 bp的片段, 其中有184个碱基出现变异. 用MEGA 3. 0软件计算的平均遗传距离为0. 089, 种间以太门哲罗鲑与布氏哲罗鲑间的遗传距离最小, 为0. 006, 推测位于黑龙江水系的太门哲罗鲑( Hucho taimen)与分布于四川岷江上游山区河流中的布氏哲罗鲑( H. bleekeri)可能为一种. 属间遗传距离以细鳞鱼属和哲罗鱼属最小, 为0. 042, 小于大麻哈鱼属内种间的遗传距离(0. 055), 推测以哲罗鱼和细鳞鱼为代表的两属间鱼亲缘关系很近. 聚类分析显示同属的鱼均能单独聚类, 是单系起源.%The mitochondrial DNA D-loop sequences of subfamily Salmoninae trout in China (10 species 5 genus) were amplified with PCR technique and sequenced to study phylogenetic relationships. After alignment, the sequence variations and genetic distance were analyzed by using MEGA 3. 0 software. There were 728 sites, among which 184 sites were varia-ble. The average genetic distance was 0. 089. The genetic distance between Hucho taimen and H. bleekeri was the smal-lest, which was 0. 006, suggesting H. Taimen located in the Heilongjiang River and H. bleekeri located in Sichuan moun-tain rivers may be the same one. Intergeneric genetic distance between Brachymystax and Hucho was the smallest 0. 042, which was less than the genetic distance ( 0. 055 ) among species of Oncrhynchus, suggesting that there was a relatively close relationship between H. Taimen and Brachymystax lenok. Fish of the same genus could be separately clustered, and they were monophyletic origin.
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