肌注和口灌氟苯尼考在吉富罗非鱼体内的药代动力学

摘要

To study the pharmacokinetics of florfenicol in,GIFT Oreochromis niloticus,after intramuscular (i.m.) and oral administrations,the fish were divided into two groups for i.m.and oral administrations.Florfenicol was administrated to tilapia at a single dose of 15 mg/kg body weight at 30 ℃.The concentration of florfenicol in the plasma was determined by HPLC method.Pharmacokinetic parameters were calculated by noncompartment modeling provided by WinNonlin 5.12 software.The results show that the absorption of i.m.was slower while the elimination was faster,the time to peak plasma florfenicol concentration (Tmax) was 4 h,the maximum plasma concentration (Cmax) was 4.64 μg/mL,the elimination half-life (T1/2λzL) was 10.45 h and the area under the concentration-time curve (AUC) in plasma was 91.06 μg·h/mL.The absorption of oral administration was faster while the elimination was slower.The time to peak plasma florfenicol concentration (Tmax) was 1 h,the Cmax was 5.92 μg/mL,T1/2λzL was 13.13 h,and the AUC was 61.96 μg·h/mL.It suggested that the absorption of oral administration was faster than that of i.m.while the elimination was slower,and the i.m.administration absorbed more completely.%通过肌内注射、口灌两种给药方式,研究氟苯尼考在罗非鱼体内的药物代谢动力学特征.把吉富罗非鱼(GIFT Oreochromis niloticus)随机分成2组,控制水温在30 ℃,以15 mg/kg分别单剂量肌内注射、口灌给药.经高效液相色谱法(HPLC)测定血浆中氟苯尼考浓度,用WinNonlin药动学软件分析药动学参数.结果表明:肌内注射氟苯尼考后,药物吸收较慢,消除较快,达峰时间(Tmax)=4 h,峰浓度(Cmax)=4.64 μg/mL,消除半衰期(T1/2λzL)=10.45 h,药-时曲线下面积(AUC)=91.06 μg·h/mL.口灌氟苯尼考后,药物吸收较快,消除较慢,Tmax=1 h,Cmax=5.92 μg/mL,T1/2λzL =13.13 h,AUC=61.96 μg·h/mL.肌内注射、口灌氟苯尼考后,二者的药动学参数差异显著,这一差异表明肌内注射给药吸收相对较慢,但更为完全(肌内注射氟苯尼考的AUC明显较大),消除相对较快.