双斑长跗萤叶甲对几种植物挥发物的触角电位和行为反应

摘要

To develop an alternative method for monitoring and controlling Monolepta hieroglyphica (Motschulsky),fourteen plant volatiles on the adult pest were screened by three methods of electrophysiological and behavioral tests.M.hieroglyphica exhibited very strong EAG responses (P ＜0.01) to peppermint essential oil diluted 102 times or 103times and to 1 mol · L-1 geraniol.In olfactometer tests,the peppermint essential oil (diluted 102 times) exhibited the best repellent effect to the M.hieroglyphica (Motschulsky),and the repelling rate was (74 ± 5.47)％ ; the geraniol (1 mol · L-1) exhibited the best lure effect to M.hieroglyphica (Motschulsky),and the luring rate was (70 ±l0.00) ％.In wind tunnel behavior tropism research,the peppermint essential oil diluted 102 times exhibited the best repellent effect to M.hieroglyphica (Motschulsky),and the repelling rate was up to (72 ± 8.37)％ ; 1 mol · L-1 geraniol exhibited the best lure effect to M.hieroglyphica (Motschulsky),and the luring rate was up to (66 ± 8.94) ％.%采用触角电位(EAG)、“Y”型嗅觉仪和风洞的方法研究双斑长跗萤叶甲成虫对14种植物挥发物的电生理和行为反应,筛选出对双斑长跗萤叶甲有效的植物挥发物,为该害虫的种群监测和防控提供新技术.研究结果表明:稀释102、103倍的薄荷精油和1 mol·L-1的香叶醇能引发双斑长跗萤叶甲强烈的EAG反应(P＜0.01),且嗅觉测试表明,薄荷精油(稀释102倍)对双斑长跗萤叶甲的最大驱避率为(74±5.47)％,香叶醇(1 mol·L-1)对双斑长跗萤叶甲的最大引诱率为(70±10.00)％.风洞行为趋性研究表明:稀释102倍的薄荷精油对双斑长跗萤叶甲的最大驱避率为(72±8.37)％,而1 mol·L-1的香叶醇对双斑长跗萤叶甲的最佳引诱率为(66±8.94)％.