高效液相色谱法测定何首乌中大黄酸、大黄素、大黄素甲醚的含量

摘要

Using 70% methanol, the Polygonum multiforum extract wasconcentrated by ultrasonic method .Then concentrated extract was leached with chloroform and separated into free and bound anthraquinones. The mobile phase was V(CH3OH):V(C3H7OH):V(H2O)=80:10:10 (control pH to 3.0 with phosphorus acid ).The HPLC system was consisted of Hypersil BDS C 18(5 μm,250nm × 46nm) and UV detector set at 439nm.The experiment results show that the average recovery of Emodin was 100.7% (RSD=1.77),and that of Emodin methylether was 98.7% (RSD=1.77%) .The relative standard deviation for Emodin acid ,Emodin and Emodin methylether were 1.18%,0.79% and 0.75% respectively.%何首乌用70％甲醇超声提取浓缩后，用氯仿萃取分离出游离和结合型蒽醌，得到定量用试液。在439nm检测波长下，以甲醇：异丙醇：水=80:10:10(磷酸调pH值为3.0)为流动相，C18填料为固定相，采用HPLC法测定何首乌中蒽醌类成分含量。平均加样回收率大黄素为100.7％，RAD=1.04.％大黄素甲醚为98.7％，RAD=1.77％％重复性实验的RAD分别为：大黄酸1.18％，大黄素0.79％，大黄素甲醚0.75％(n=10)。