硝酸银诱导不同大豆品种中大豆抗毒素生成量的差异

摘要

目的：研究硝酸银对不同黄豆、青豆和黑豆诱导大豆抗毒素生成量的比较。方法：以0.01mol/L硝酸银为诱导剂,采用分析型高效液相法测定9种不同品种大豆产生大豆抗毒素的生成量。应用C18反相色谱柱（4.6mm×250mm,5μm）,以乙酸水溶液（pH3）和乙腈为流动相,进行梯度洗脱,流速1mL/min,柱温40℃,检测波长285nm。结果：在相同的提取和测定条件下,硝酸银诱导大豆生成大豆抗毒素的生成量由高至低顺序为青豆、黑豆、黄豆。结论：本研究结果可为合理选择不同种类的大豆进行大豆抗毒素的研究和应用提供依据。%Objective: To compare the changes in silver nitrate-induced glyceollin production in three types of soybeans: yellow,green and black,each of which consisted of three cultivars.Methods: Glyceollin production in soybeans was induced by 0.01 mol/L silver nitrate solution and determined by HPLC.The chromatographic separation was performed on a C18 column(4.6 mm × 250 mm,5 μm) using a mobile phase consisting of acetic acid solution(pH 3) and acetonitrile at a flow rate of 1 mL/min through gradient elution.Glyceollin was detected at 285 nm.Column temperature was set at 40 ℃.Results: Under the same extraction and determination conditions,silver nitrate-induced glyceollin production in soybeans decreased in the following order: green black yellow.Conclusion: The results obtained in this study can provide evidence for the selection of suitable soybean type for research and applications of glyceollins.