The composition and structure of glycerides in chicken fat were analyzed by reversed-phase high performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC-APCI MS) and reversed-phase high performance liquid chromatography with an evaporative light-scattering detector (RP-HPLC-ELSD). The chromatographic separation was performed on a Cls using a mobile phase of acetonia'ile-methylene chloride (60:40, V/V). Glycerides were monitored by APCI MS in positive mode. According to [M+H]+ and [M+H - RCOOH]+, 26 glycerides were identified. Based on the abundance of [M+H-RCOOH] + and ion fragments from collision induced dissociation (CID) of ion trap, the distribution of fatty acids in glycerides was deduced. Triacylglycerols represented 99.88% of total glycerides as demonstrated through RP-HPLC- ELSD analysis and quantification by peak area normalization. Among these triacylglycerols, 1-palmitoyl-2,3-di-oleoyl-glycerol revealed the highest content (23.65%), followed by 1-palmitoyl-2-oleoyl-3-linoleoyl-glycerol (19.37%), and 1-1inoleoyl-2,3-di- oleoyl-glycerol (16.02%).%利用反相高效液相色谱．大气压化学电离质谱（RP－HPLC－APCIMS）及反相高效液相色谱－蒸发光散射检测（RP－HPLC－ELSD）分析鸡脂甘油酯组成及结构。C18色谱柱，乙腈．二氯甲烷（60：40，V／V）为流动相，APCIMS正离子模式。根据APCI－级质谱的准分子离子及二酰基甘油碎片离子鉴定出26种甘油酯，包括4种甘油二酯和22种甘油三酯。根据二酰基甘油碎片离子的丰度高低及离子阱碰撞诱导解离产生的二级／三级碎片离子的丰度高低，判定各甘油酯中脂肪酸的位置分布。采用RP－HPLC－ELSD分析，面积归－化法定量，甘油三酯占总甘油酯的99．88％，含量最高的为1．棕榈酸－2,3－二油酸甘油酯（23．65％），其次为1－棕榈酸－2－油酸－3－亚油酸甘油酯（19．37％）、1-亚油酸－2，3－二油酸甘油酯（16．02％）。
展开▼
