糯米藤多糖的提取工艺优化

摘要

Objective： To find optimal conditions for the extraction of polysaccharides from whole plants of Gonostegia hirta （BL;） Miq. Methods： Solvent reflux extraction was used for polysaccharide extraction. Five parameters that influence extraction efficiency were investigated using one-factor-at-a-time and orthogonal array design methods, and three main ones were opti- mized based on a quadratic rotation-orthogonal composite experimental design. Results： In decreasing order of their effect on extraction efficiency, five influential parameters were ranked as extraction temperature 〉 solvent-to-solid ratio 〉 extraction time 〉 extraction number 〉 soaking time. Their optimal levels were 96.26 ℃, 27.28： 1 （mL/g）, 109.20 min each time, 2 and 90 min, respectively, resulting in an extraction yield of 25.121%. Conclusion： Quadratic rotation-orthogonal composite experimen- tal design permits reasonable optimization of polysaccharide extraction from Gonostegia hirta （BL.） Miq.%目的：研究提取糯米藤多糖的最佳工艺条件。方法：以多糖提取率为考察指标，溶剂回流作为提取手段，利用单因素及正交试验确定影响提取的主要因素，然后使用回归正交组合设计优化提取工艺。结果：影响糯米藤多糖提取率的因素顺序为提取温度〉液固LL〉提取时间〉提取次数〉浸泡时间；回流提取的最佳工艺条件为在96．26℃水浴条件下，提取时间109．20min、提取次数2次、液固比27．28：1（mL／g）、浸泡时间90min，最优工艺条件下多糖提取率可达25．121％。结论：使用回归正交设计能合理地优化糯米藤多糖的提取工艺。