灰树花多糖具有抗肿瘤、抗HIV、抗氧化等作用,为探明灰树花多糖合成的遗传基础,采用Illumina高通量测序技术对灰树花转录组进行测序,获得74 575 910个reads,10.4 G数据量;将测序数据进行de novo拼接后得到18 077个Unigene.对所得Unigene进行不同数据库比对,在Nr数据库中有11 651个Unigene被注释到,其中灰树花转录组与变色栓菌相似序列最多(18.74％);有8 332个Unigene在GO数据库中注释到,分为细胞组分、分子功能及生物学过程等3大类59个分支;与KEGG数据库比对,共有5 200个Unigene被注释,分属376条代谢通路.从18 077个Unigene中共找到1 155个简单重复序列(simple sequence repeat,SSR)位点,其中单核苷酸重复最高,六核苷酸重复最少,A/T出现频率最高.本研究中在KEGG数据库注释到115个Unigene与灰树花多糖合成有关,这些Unigene及其注释信息为今后深入开展灰树花多糖代谢途径及相关功能基因等研究提供了依据.%The exopolysaccharide of Grifola frondosa possesses anti-tumor,anti-HIV,and anti-oxidant effects.In order to investigate the genetic basis for polysaccharide synthesis by G.frondosa,the transcriptome of G.frondosa was sequenced by high-throughput sequencing technology.A total of 74 575 910 reads and 10.4 G data were generated,which formed 18 077 Unigenes after de novo splicing.A total of 11 651 Unigenes were annotated in the Nr database.Among them,G.frondosa transcriptome had the most similar sequence (18.74％) to M.tumefaciens.A total of 8 332 Unigenes were annotated in the GO database,which were divided into 3 major categories:molecular composition,molecular function and biological processes,including 59 branches.Compared with the KEGG database,5 200 Unigenes were annotated which belonged to 376 metabolic pathways.A total of 1 155 simple sequence repeat (SSR) loci were found in 18 077 Unigenes,among which the highest repeat was single nucleotide whereas the repetition of hexanucleotide was the least,and the frequency of A/T was the highest.In this study,115 unigenes which have been annotated in the database of KEGG were related to the biosynthesis of polysaccharide by G.frondosa.These annotated Unigenes and the information about them can lay the foundation for further studies on polysaccharide metabolism pathways and related functional genes in G.frondosa.
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