液体磁流芯片检测牛白血病方法的建立

摘要

Using Peptide coupling immunomagnetic beads, detection method of magnetic fluid chip was established by screening and optimizing blocking solutions, serum diluent and HRP-labeled rabbit anti-cow IgG. Forty-one serum of bovine were detected by methods of magnetic fluid chip and foreign kit. The results showed that the coincidence rate of positive serum and negative were 100%and 97.1%respectively.%采用gp51肽偶联免疫磁珠为材料，通过对封闭液、血清稀释浓度和HRP标记的兔抗牛IgG工作浓度的筛选和优化，建立了针对牛白血病的液体磁流芯片检测方法。运用本方法和国外试剂盒法对41份牛血清进行检测，结果表明，两种方法检测到的阳性血清符合率为100%，阴性血清符合率为97.1%。