高效液相色谱法测定金银花中绿原酸的含量

摘要

建立金银花中绿原酸含量测定标准.用HPLC方法测定,以水-甲醇-乙腈-冰醋酸(95:10:5:2)为流动相, 327 nm为检测波长.绿原酸平均回收率为97.36%,RSD为0.86%,线性范围为0.107μg/mL~0.642μg/mL.本方法灵敏、准确、稳定可靠、重复性好.%To establish a concentration measurement standards of Weight loss granule. HPLC methord. Water-Methyl alcohol-glacial acetic acid(95:10:5:2)was used as the mobile phase and UV detector was at 327 nm. The average recovery rate of chlorogenic acid is 97.36%and RSD is 0.86%.Linear range is 0.107 μg/mL-0.642μg/mL.The methord is rapid,accurate,steady and reliable and the repetition is good.