高效液相色谱法检测运动饮料中的L-肉碱

摘要

建立一种快速、有效的高效液相色谱法测定运动饮料中L-肉碱的方法.样品经1.0 mmol/L盐酸提取后,采用MCX固相萃取小柱进行净化和富集.采用RP C18柱(4.6 mm×250 mm,5μm)分离,以乙腈:辛烷磺酸钠(50 mmol/L)=25:75(体积比)作为流动相,用PDA检测器检测,外标法峰面积定量.结果表明,L-肉碱标液在1.0μg/mL~20.0μg/mL浓度范围内线性良好,相关系数r2为0.999,检出限为0.1 mg/kg,定量限为0.3 mg/kg,加标回收率达到82.3%~84.1%.%A rapid and effective method was established for the determination of L-carnitine in sports drink by HPLC. After the sample was extracted by 1.0 mmol/L HCl, it concentrated and purified by MCX solid phase ex-traction column. The separation of targeted compound was performed on RP C18 column(4.6 mm×250 mm, 5 μm) using acetonitrile:sodium octane sulfonate (50 mmol/L)(25:75,volume ratio)as mobile phase, with PDA detector and external standard method peak area quantification. The linear range of L-carnitine was in the range of 1.0μg/mL-20.0μg/mL with a correlation coefficient of 0.999. The detection limit was 0.1 mg/kg and the quantitative limit was 0.3 mg/kg.The recovery rate was 82.3%-84.1%.