分光光度法测定铁质叶酸片中铁元素的含量

摘要

A method for the determination of iron content in health food tablets by ultraviolet spectrophotometry was established. Use 50% of the hydrochloric acid solution and 10% of hydroxylamine hydrochloride processing samples to make all the iron in samples change into Fe2+. Fe2+ can react with 0. 15% of the phenanthroline solution to form a stable orange red compound. Then all the iron can be deteminated by spectrophotometric method on 511 nm. Results: Fe2+ was good linear over the range 0. 022 4～0. 112 0 mg/mL, the average recovery of Fe2+ was 99. 2%, and RSD was 1% (n=6). This method is simple,stable,precise and accurate. So this study can be used in determination of iron content, in iron folic acid tablets.%建立测定铁质叶酸片中铁含量的方法.用1∶1的盐酸溶液和10％的盐酸羟胺处理样品,使样品中的铁全部转化成Fe2+,Fe2+与0.15％的邻菲罗啉显色剂络合生成稳定的橙红色化合物,按分光光度法,在511 nm处测定吸光度.结果表明,铁离子浓度在0.022 4～0.112 0 mg/mL范围内呈现良好线性.平均回收率为99.2％.RSD为1％(n=6).该方法操作简单,样品前处理简便,结果稳定、精确,适用范围广,可用于铁质叶酸片中铁元素含量的测定.