赤藓红与牛血清白蛋白的相互作用及金属离子的影响

摘要

赤藓红具有潜在致癌性,被美国等国家禁止在食品中添加.本文采用荧光光谱法和紫外光谱法研究了赤藓红与牛血清白蛋白(BSA)的相互作用机制及金属离子Cu2+和Zn2+对其影响.实验结果表明:赤藓红和BSA复合物的形成导致了BSA内源荧光的猝灭;赤藓红与BSA之间主要作用力为静电作用力;依据F?ster非辐射能量转移理论求得赤藓红与BSA之间的结合距离为4.81nm.Cu2+和Zn2+的参与使赤藓红-BSA的猝灭常数增大;Zn2+的参与减小了赤藓红-BSA的结合常数,且改变了赤藓红与BSA之间作用力类型.%Erythrosine (Ery) have been banned in food by USA because its potential carcinogenicity. The interac-tion between Ery and bovine serum albumin (BSA) was studied by fluorescence spectrum and UV-vis spectrum. The effect of metal ions (Cu2+, Zn2+) on the BSA-Ery system was also researched. The results indicated that the formation of Ery-BSA complex caused fluorescence quenching of BSA. The interaction of Ery and BSA were elec-trostatic interaction. The distance r between Ery and BSA was 4.81nm based on F?ster's non-radiative energy transfer theory. Cu2+ and Zn2+ increased the quenching constants of Ery with BSA, Zn2+ reduced the binding con-stants of Ery with BSA, and the binding force changed to hydrogen bonds and van der Waals' forces.