一株非谷氨酸依赖型聚谷氨酸产生菌的筛选和鉴定

摘要

A strain SK19. 001 which could produce poly γ-glutamate （γ-PGA） independent of glutamic acid in culture was isolated. It was identified as Bacillus methylotrophicus based on the sequence analysis of 16S rDNA and a series of morphological and biochemical characteristics. The yield of -y-PGA was 14.57 g/L when the strain was grown in glycerol and peptone F403-containing medium at 37℃ for 24 h with shaking.%从土壤中分离到1株可不依赖谷氨酸发酵产γ-聚谷氨酸（γ-PGA）的菌株SK19．001，通过16SrDNA序列比对并结合菌株的形态、生理生化特征分析，鉴定此菌株为甲基营养芽孢杆菌（Bacillus methylotrophicus）。在以甘油和蛋白胨F403为碳源和氮源的培养基中，37％振荡培养24h，γ-PGA产量可达到14．57g／L。