大孔树脂纯化马兰总黄酮树脂吸附特性及工艺的研究

摘要

研究大孔树脂纯化马兰总黄酮树脂吸附特性及工艺条件及参数。文中分别进行静态吸附、静态解吸、静态吸附动力学过程（Lagergren准一级动力学方程）、静态吸附等温曲线（Langmuir和Freundich等温吸附方程）、动态吸附实验,从7种大孔树脂中筛选用于马兰总黄酮分离的最佳树脂,并系统研究最佳大孔树脂分离纯化的吸附性能和最优洗脱参数。结果表明：D101型大孔树脂为分离马兰黄酮类组分最佳树脂,其分离的最佳工艺为总黄酮浓度为9.36 mg/mL的样液,以3 BV/h的流速,控制pH值为4～5上柱,用75%乙醇以3 BV/h用量进行洗脱,可获得样品总黄酮纯度达70%以上。%To investigate the adsorption characteristics and purification process of the flavonoids from Kalimeris indica by macroreticular resin.The static capacity absorption,static capacity desorption,static adsorption dynamics（quasi-order kinetic equation of Lagergren）,static adsorption isothermal（Langmuir and Freundich）,dynamic absorption ratio and dynamic elution ratio of seven types of macroreticular resin were studied and compared in order to find the best one,and the adsorption and desorption performances of the best one were studied in detail.The type of D101 resin gave the best effect of separation with the sampling rate 3 BV /h and concentrations of 9.36 mg/mL in pH 4-5,and the concentration of elution was 75 % ethanol with eluting velocity 3 BV/h.The purity of total flavonoids in the product was up to 70%,and the purification process was feasible.