‘神农菊’挥发油提取优化及其在牛奶中的抑菌性能

摘要

To achieve the optimization of the extraction technology of volatile oil from the Chrysanthemum morifolium cv Shennongju,the single-factor experiment and the Box-Benhnken experimental were used,and the results of the experiments were analysed by the response surface analysis method.Furthermore,the antibacterial activity of volatile oil in the milk was studied.The results show that the optimization of the extraction technology of volatile oil from the C.morifolium cv Shennongju was 5.18 h of the extraction time,1 ∶ 14.15 of the material to liquid ratio,16.58％ of the concentration of sodium choride.According to the Second-degree polynomials regression equation,the predicted value of the extraction rate of the volatile oil was 0.566％;And base on the optimal extraction condition,the reality extraction rate of the volatile oil was 0.559％,which the error to the theoretical value was 1％ approximate.In contrast,the amounts of bacterium in the milk,with the volatile oil,were more less than these in the check tests.With the increase of volatile oil,the antibacterial activity in the milk is increased.%应用单因素与Box-Benhnken试验,结合响应面分析法,对‘神农菊’挥发油最佳提取条件进行了相关分析研究;同时,以牛乳中的活菌数量为衡量指标,对‘神农菊’挥发油的抑菌性能做了体外试验.实验结果表明:‘神农菊’挥发油的最佳提取工艺为提取时间5.18h,料液比为1∶14.15,NaCl浓度为16.58％,该条件下挥发油得率的预测值为0.566％;在调整后的最佳提取条件下,实际挥发油得率为0.559％,与理论值的误差约为1％;加入‘神农菊’挥发油的牛乳中的活菌数量远远小于对照组中的活菌数量,且挥发油的抑菌性能随着其浓度的增加而增强.