分光光度计比浊法测定抗菌物质效价

摘要

Agar diffusion method is widely used for determine the antimicrobial activities,but errors due to human factors always influence the accuracy of the results.The objective of this study was to set up a spectrophotometer turbidimetry with characteristics of accuracy,simpleness,and no special requirement for equipments to determine the potency of all kinds of antimicrobial substances.Results revealed that the calibration curves of three antimicrobial substances,including bacteriocin nisin,bacteriocin-like substance NFL and antibiotic gentamycin sulfate,were linear in the experimental range at the sampling time point which corresponded to the maximal inhibitory rate with middle inoculum size (R2 ＞ 0.99).This method is suitable for precise determination of all kinds of antimicrobial substances under simple laboratory conditions.%琼脂扩散法是测定抗菌物质效价常用的方法,但人为操作因素常常影响实验结果的准确性.实验旨在建立一种测定准确、操作简单、无需特殊设备的分光光度计比浊法来测定各种抗菌物质的效价.研究发现,以抑菌率最高时对应的培养时间为取样时间点,在中等接种量下,在实验浓度范围内,细菌素nisin、类细菌素NFL和抗生素硫酸庆大霉素这3种抗菌物质的浓度与抑菌率之间的线性关系良好,R2值均高于0.99,标准曲线成立.该法适用于设备条件简单的实验室准确测定各类抗菌物质效价.