聚三聚氰胺修饰玻碳电极测定食品中叔丁基对苯二酚

摘要

采用电化学聚合方法制备了聚三聚氰胺膜修饰玻碳电极(poly melamine film modified glassy carbon electrode,PMeL/GCE),并采用循环伏安法研究了抗氧化剂叔丁基对苯二酚(tert-butylhydroquinone,TBHQ)在修饰电极上的电化学行为,发现其氧化还原峰电流较在玻碳电极(glassy carbon electrode,GCE)上明显增强,提高了检测的灵敏度.对三聚氰胺电化学聚合时间、扫描速率、电压、段数以及测定TBHQ溶液的pH值等实验条件进行了优化.在最佳的条件下,TBHQ在4.00 ×l0-7～ 1.00×10-4mol/L内与其氧化峰电流呈线性关系,相关系数:R=0.997 5,检出限为2.00×10-9mol/L.将修饰电极用于食品中TBHQ的检测,回收率在96.9％ ～ 102.2％之间.%Poly melamine film modified glassy carbon electrode (PMel/GCE) was fabricated by electroplymerization of melamine.The electrochemical behavior of antioxidants tert-butylhydroquinone (TBHQ) was investigated by cyclic voltammetry (CV),a couple of sensitive redox peaks obtained.It was found that the oxidation peak current (ipa) and the reductive peak (ipc) current at PMel/GCE were obviously stronger than those at the bare glassy carbon electrode(GCE),which indicated that the sensitivity for detection of TBHQ was improved.Experimental parameters,including electro-polymerized time of Mel,scan rate,voltage,cycles and pH value of buffer solution were optimized.Under the optimal conditions,good liner relationships between current intensities and TBHQ concentrations were obtained in the ranges of 4.00 × 10-7-1.00 × 10-4 mol/L,with correlation coefficients (R) of 0.997 5.The detection limit was 2.0 × 10-9 mol/L.The modified electrode can be applied to the analysis of TBHQ in food with recoveries of 96.9％-102.2％.