柠檬烯纳米脂质体的制备及其性质测定

摘要

以柠檬烯为原料,利用乙醇注入法制备柠檬烯纳米脂质体.在单因素试验基础上,以胆固醇添加量、柠檬烯添加量、磷酸盐缓冲溶液(phosphate buffer solution,PBS)的温度为影响因素,以包封率为响应值,根据Box-Behnken试验设计原理,采用3因素3水平的响应面分析法优化柠檬烯脂质体的制备工艺,得到柠檬烯脂质体的最佳制备工艺条件为:胆固醇的添加量为8.8 mg、柠檬烯添加量为12.7 mg、PBS温度为51℃,在此条件下柠檬烯纳米脂质体的包封率为(67.44±0.58)％,与模型预测值相对误差为0.4％,重现性良好,平均粒径为(165.4±2.08)nm,PDI为(0.185±0.011),Zeta电位值为(-16.23±0.569)mV.%In this experiment,limonene lipsomes were prepared by ethanol injection method with limonene as the material.One-factor-at-a-time design was used to investigate the effects of six process parameters on encapsulation efficiency.Three main process parameters that influence encapsulation efficiency including the cholesterol content,the limonene content and the temperature of phosphate buffer solution were optimized by response surface analysis based on a three-variable,three-level Box-Behnken experimental design.The optimal conditions for preparing limonene liposomes were determined as the amount of cholesterol 8.8 mg,limonene 12.7 mg,and the temperature of PBS 51℃.Under these conditions,the actual encapsulation efficiency of limonene liposomes was (67.44 ± 0.58) ％,which showed a relative error of 0.4 ％ when compared with the predicted value.The average particle size was (165.4 ± 2.08) nm,PDI was (0.185-±0.011),Zeta potential was (-16.23 ±0.569) mV.