I型胶原蛋白纳米纤维膜的制备及结构表征

摘要

Objective To prepare typeⅠcollagen nanofiber membrane and characterize its structure. Methods Bovine achilles tendon collagen was dissolved in 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) and dilute acetic acid, respectively, to form spinning solution. The collagen nanofiber membrane was made of collagen and polyethylene oxide (PEO) through electrospinning. The microscopic morphology and structure of nanofibers were investigated by scanning electromicroscopy, infrared spectroscopy and thermal analysis. Results PEO improved obviously the spinnability of solution and the morphology of collagen nanofibers, and the microstructure and thermal properties of collagen did not change too much before and after electrospinning. Conclusion The nanofiber membrane prepared through electrospinning from bovine achilles tendon collagen can lay the foundation for the further application of collagen nanofiber membrane, such as medical dressing for wound healing.%目的：制备Ⅰ型胶原蛋白纳米纤维膜及对其进行结构表征。方法以牛跟腱胶原蛋白为原材料，分别以六氟异丙醇（HFIP）和稀醋酸为溶剂制备纺丝液，添加聚氧化乙烯（PEO）提高可纺性，在适当的纺丝条件下进行静电纺丝制备胶原蛋白纳米纤维膜。通过扫描电镜观察纳米纤维的微观形态，采用红外光谱和热重分析表征纳米纤维的细微结构的变化。结果加入PEO能显著提高胶原蛋白可纺性，改善纳米纤维的微观形态，静电纺丝前后胶原蛋白的热力学性能和微观结构无变化。结论牛跟腱胶原蛋白能通过静电纺丝制成纳米纤维膜，为进一步利用胶原纳米纤维膜制备促伤口愈合医用敷料提供了支持。