高效液相色谱测定清肝颗粒中胡黄连苷Ⅰ和胡黄连苷Ⅱ含量的研究

摘要

目的 建立清肝颗粒中胡黄连苷Ⅰ和胡黄连苷Ⅱ含量测定方法.方法 采用高效液相色谱法(HPLC),Agilent Zorbax Extend C18色谱柱(4.6 mm×150 mm,5μm),柱温35℃,流动相为乙腈-水-磷酸(13:87:0.1),流速1.0 mL/min,检测波长275 nm,进样量10μL.结果 胡黄连苷Ⅰ在6.36~127.20μg/mL范围内呈现良好线性关系(r=1.0000),胡黄连苷Ⅱ在16.16~323.20μg/mL范围内呈现良好线性关系(r=1.0000);胡黄连苷Ⅰ的平均加样回收率为97.58%(n=6),RSD为1.41%,胡黄连苷Ⅱ的平均加样回收率为96.63%(n=6),RSD为1.64%.结论 该方法操作简便,准确,稳定,灵敏度高,适用于清肝颗粒的质量控制.%Objective To establish a method for the determination of the contents of picroside Ⅰ and picroside Ⅱ in Qinggan Granules by HPLC. Methods The contents of picroside I and picroside Ⅱ in Qinggan Granules were determined by HPLC with Agilent Zorbax Extend C18 (4.6 mm×150 mm, 5 μm) chromatographic column, using acetonitrile-water-phosphoric acid (13:87:0.1) as mobile phase. The flow rate, detection wave length and column temperature were set at 1.0 mL/min, 275 nm and 35 ℃, respectively. Results The linear relationship was good in the range of 6.36-127.20 μg/mL for picroside Ⅰ (r=1.0000) and 16.16-323.20 μg for picroside Ⅱ (r=1.0000). The average recovery for picroside Ⅰ was 97.58 %, with RSD of 1.41 % (n=6). The average recovery for picroside Ⅱ was 96.63 %, with RSD of 1.64 % (n=6). Conclusion This method is simple, accurate, stable and sensitive, thus can be applied in quality control of Qinggan Granules.