Experimental Studies of the Effect of Forskolin on the Lowering of Intraocular Pressure

摘要

Purpose:To determine the effect of domestic Forskolin on lowering of intraocular pressure(IOP)in rabbits.Methods:Measure the normal IOP and ocular hypertension by water load in rab-bits using Perkins tonometer.Measure the adenylate cyclase(AC)activity by Ho &Sutherland'2 method.Results:The results show that Forskolin significantly lowered the normalIOPof rabbits and blocked the ocular hypertension induced by water logad in rabbits(P<0.01).The maximum decrease value of 2%.1%and0.5%of the Forskolin was0.59,0.36and0.19kPa(1kPa=7.5mmHg),which showed the noticeable dose-effect relationship.Topical ocular appliccation of Forskolin lowered IOP in half an hour,reached to a peak in 2-3hours and remained significantly for10hours The pupillary diameter did not change when IOP was reduced.The Forskolin had po-tent stimulative properties to AC.The greater th ability of the Forskolin to stimulate AC,the stronger the effect of IOP lowering.Conclusions:The Forkolin had the effect on lowering the IOP of rabbits.The mechanism of IOP reduction by Forskolin is related to its AC activation.Eye Sci-ence1995;11:173-176.