超声波破碎仪在高通量测序中的应用

摘要

Data quality control in high-throughput sequencing technology is an important problem in the sequencing process. The good sample library is the prerequisite for high-quality sequencing results. In particular, the process of sample fragments has still been under the spotlight. This paper preliminarily compares non-contact automatic ultrasonic disrupter Bioruptor with high performance Ultra-Sonicator Covaris S220 in utilization skills and their pros and cons involved in the fragmentation technique of genomic DNA samples.%高通量测序技术中的数据质量控制是测序过程中的重要问题,样本文库的质量则是获取高质量的测序数据的前提和基础.在各种样本文库的制备过程中,样本的片段化过程受到普遍关注.该文主要针对基因组DNA样本的片段化技术中,涉及到的非接触式全自动超声破碎仪Bioruptor和自动聚焦声波样本处理仪Covaris S220的使用技巧和仪器的优缺点进行了初步比较.