鼻咽癌患者外周血淋巴细胞中EB病毒DNA载量与临床分期的关系研究

摘要

Objective To detect Epstein-Barr virus DNA load in peripheral blood lymphocytes of nasopharyngeal carcinoma (NPC) patients, and analyze its relationship with the clinical stage of NPC. Methods Real-time quantitative PCR was used to detect EB virus DNA in peripheral blood lymphocytes of 87 NPC patients in our hospital from January 2012 to January 2013, and relationship between the DNA load and NPC clinical stage was analyzed. Results The positive rate of EBV-DNA in NPC patients was 82.76%(72/87) , and a median level of EBV-DNA was 7.58×106copies/ml. The copy numbers of EBV-DNA for stage I, Ⅱ,ⅢandⅣof NPC were (4.25±1.14)×103, (4.56±0.68)×104, (7.88±1.65)×105, and (8.09±1.65)×106, respectively, showing a gradually in-creased tendency from stage I to stage Ⅳ, and the difference was significant (P<0.05). Conclusion EBV-DNA load in peripheral blood lymphocytes is positively correlated with the clinical stage of patients with NPC ,suggesting that EBV-DNA load is closely related to NPC progress, and it can be used as an effective indicator of disease monitoring and efficacy judgement.%目的：检测鼻咽癌患者外周血淋巴细胞中EB病毒DNA载量，并分析其与鼻咽癌临床分期的关系。方法选取2012年1月至2013年1月我院肿瘤科收治住院的87例鼻咽癌患者，采用实时荧光定量PCR法检测外周血淋巴细胞EB病毒DNA含量；统计学分析其与患者临床分期的关系。结果鼻咽癌患者外周血淋巴细胞中EB病毒DNA检出率为82.76%(72/87)，EB病毒DNA拷贝数的中位数为7.58×106拷贝数/ml，Ⅰ、Ⅱ、Ⅲ和Ⅳ期患者每毫升DNA拷贝数分别为(4.25±1.14)×103、(4.56±0.68)×104、(7.88±1.65)×105和(8.09±1.65)×106；EB病毒DNA载量随着疾病临床分期的增加呈现逐步升高的趋势，且差异显著(P<0.05)。结论鼻咽癌患者外周血淋巴细胞中EB病毒DNA载量与患者临床分期相关，提示EB病毒DNA载量与鼻咽癌病情进展关系密切，在临床上可作为鼻咽癌患者病情监测及判断疗效的一种有效手段。