稀脉萍对水中甲基橙的毒害响应及去除研究

摘要

Methyl orange was used as an environmental stress factor to examine the effects on enzyme activity of peroxidase,content of soluble protein,and chlorophyll content of Lemna aequinoctialis leaves.L.aequinoctialis was applied to removal Methyl orange.The results showed that enzyme activity of peroxidase rised first and then fell with the increase of Methyl orange concentration.When Methyl orange concentration was 1 40 mg/L,enzyme activi-ty of peroxidase came to the maximum.When Methyl orange concentration was more than 1 00 mg/L,soluble pro-tein content dropped rapidly.The chlorophyll content and the ratio of chlorophyll a to chlorophyll b decreased with the increase of Methyl orange concentration.When Methyl orange concentration was 20 mg/L,there was a 1 0 -day lag period.Then the degradation rate of Methyl orange could reach 89.6 %after L.aequinoctialis was cultured 24 days.%用甲基橙作为胁迫因子，研究了甲基橙对稀脉萍叶片过氧化物酶活性、可溶性蛋白含量和叶绿素含量的影响，并采用稀脉萍去除甲基橙。结果表明：随着甲基橙浓度的增加，过氧化物酶活性呈先上升后下降的趋势，当甲基橙浓度为140mg／L时，过氧化物酶活性达到最高值。当甲基橙浓度＞100mg／L，可溶性蛋白含量急速下降。叶绿素含量和叶绿素a／b比值均随甲基橙浓度的升高而降低。当甲基橙浓度为20 mg／L时，稀脉萍对甲基橙的去除需经10 d的延滞期，24 d后去除率达89.6％。