DNPH 衍生化采样－溶剂解析－高效液相色谱法同时测定木制品中25种醛酮化合物

摘要

The measurement of aldehydes and ketones in wood materials was established using 2,4-dinitrophenylhydrazine (DNPH) derivatization sampling and solvent desorption coupled with high performance liquid chromatography ( HPLC) .A SEP-PAK column was attached to the front of the DNPH sampling column .Sample volume and flow speed were maintained below 10 L and 400 mL/min, re-spectively.The method showed good separation between 25 aldehydes and ketones , with good linearity in the range of 0.005 ~3 mg/L.The correlation coefficient R2 was above 0.999 6, and the recovery rates and precision were in the range of 85.3%~113.5%and 2.67%~4.56%, respectively.When the sampling volume was 10 L, the detection limit and quantitative limit of 25 aldehydes and ketones were less than 0.16 and 0.50 μg/m3 , respectively.%建立了DNPH衍生化采样－溶剂解析－高效液相色谱测定木制品中醛酮化合物的方法。采样时需在2，4－二硝基苯肼吸附管前串联一只SEP－PAK脱臭氧小柱；采样体积应≤10 L，流量≤400 mL／min。该法对25种醛（酮）化合物对应的衍生物分离效果良好，在0．005～3 mg／L范围内线性良好，相关系数R2≥0．9996，回收率为85．3％～113．5％，精密度为2．67％～4．56％。采样量为10 L时，25种醛（酮）化合物的检出限和定量限分别≤0．16和0．50μg／m3。