紫外-可见分光光度法快速确定细菌菌液的浓度

摘要

Objective:To establish an UV-Vis Spectrophotometric method for bacterial counts.Methods: UV-Vis spectrophotometry and turbidimetry were used to investigate the correlations between two methods and absorbance with concentration of bacteria counts .Results:The correlation of absorbance with concentration of bacteria was well.The results of bacterial counts were stable.Conclusion:UV-Vis spectrophotometric method is rapid and suitable for bacterial counts.%目的：建立快速确定细菌菌液浓度的方法。方法：采用紫外-可见分光光度法和比浊法进行细菌计数，考察两种方法的相关性以及菌液浓度与吸光度之间的相关性。结果：细菌菌液的吸光度与其菌液浓度之间相关性良好，相应菌落计数结果稳定。结论：紫外-可见分光光度计可用于细菌菌液的快速计数。