实时定量PCR法分析2型糖尿病患者肠道乳酸杆菌菌种数量改变分析

摘要

Objective to type 2 diabetic patients with intestinal Lactobacillus bacteria quantity change situation was analyzed by real-time quantitative PCR analysis.Methods in our hospital 100 cases of type 2 diabetes mellitus patients as the research object, and 100 healthy volunteers as than, their blood glucose, blood lipid levels were detected, and the collection of stool specimens of extracting bacteria DNA, do the quantitative real-time PCR amplification experiments, comparing the total lactic acid bacillus and the specific bacteria on two groups of experimenters quantitatively.Results the indexes of blood glucose, blood lipids of diabetic patients were significantly higher than those in healthy volunteers, the comparison of the total lactic acid bacteria except Lactobacillus plantarum abroad and Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus casei, type 2 diabetes mellitus patients also were higher than those in healthy volunteers, the comparison were statistically significant difference (P<0.05). There was negative correlation between lactobacilli and low density lipoprotein cholesterol,P<0.05, with statistical significance.Conclusion intestinal type 2 diabetic patients in the amount of Lactobacillus strain abnormally increased, suggesting that in patients on treatment time can adjust the number of Lactobacillus strains improved glucose, lipid metabolism disorders in patients with.%目的：通过实时定量PCR法分析2型糖尿病患者肠道乳酸杆菌菌种数量改变情况进行分析。方法选取该院收治的2型糖尿病患者100例作为研究对象,并以100例健康志愿者作比,分别检测其血糖、血脂水平,并采集粪便标本提取细菌DNA,做实时定量PCR扩增实验,对两组实验者的总乳酸杆菌及具体菌种进行定量比较。结果糖尿病患者各项血糖、血脂指标均显著高于健康志愿者,除植物乳杆菌外的总乳酸杆菌及嗜酸乳杆菌、保加利亚乳杆菌、鼠李糖乳杆菌、干酪乳杆菌比较,2型糖尿病组患者也均高于健康志愿者,比较均差异有统计学意义(P<0.05)。乳酸杆菌与低密度脂蛋白胆固醇之间呈负相关性,差异有统计学意义(P<0.05)。结论2型糖尿病患者肠道内乳酸杆菌菌种数量异常增加,提示在对患者进行治疗的时候可以通过饮食调节乳酸杆菌菌种数量改善患者的血糖、血脂代谢失调。