The gene of Helicobacter pylori can encode three to four type IV secretory systems,of which a new gene region has been found in the H.pylori plasticity region.The coding products of this region can form a new T4SS named tfs3,but its function is unclear.This study investigated the effect of VirD4 recombinant protein in the tfs3 secretory system of the H.pylori clinical strain SBK on GES-1 cells.We observed changes in cell morphology after VirD4 treatment.Further analysis indicated that VirD4 increased inflammation by increasing the activation of NF-κB.VirD4 can also inhibited proliferation,and induced migration of cells.Moreover,VirD4 caused apoptosis in GES-1 cells in caspase and ERK1/2/Ras dependent signaling events.Our study laid a foundation for further research on the biological function of VirD4 and the detection and treatment of H.pylori-related diseases.
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