A HPLC method for determination of three impurities in sulfamonomethoxine was established. A CLC-ODS column was used with the mobile phase of 0.05mol/L KH2PO4 solution- acetonitrile (86∶14) at the detection wavelength of 256nm. The calibration curves of three impurities were linear (r>0.999) in the concentration ranges of 3 - 240μg/ml, 3 - 120μg/ml and 3 - 60μg/ml, respectively. The average recoveries were 101.1%, 102.2% and 102.8%, respectively. The detection limit of three impurities was 100ng/ml.
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