Malvidin-3-galactoside from blueberry suppresses the growth and metastasis potential of hepatocellular carcinoma cell Huh-7 by regulating apoptosis and metastases pathways

摘要

Malvidin-3-galactoside(Mv-3-gal)is the major anthocyanin monomer in blueberry anthocyanins.The compound is well-characterized by its anti-oncogenesis function in multiple organs including liver.In the current study,the mechanism driving the anti-hepatocellular carcinoma(HCC)function of Mv-3-gal was further explored by focusing on apoptosis and metastases pathways.HCC cell line Huh-7 was firstly administrated with Mv-3-gal of different concentrations and the effect of Mv-3-gal on cell proliferation,apoptosis,colony formation ability,metastasis potential as well as the activity of Akt/PTEN and MAPK pathways was assessed.The results showed that Mv-3-gal inhibited the cell proliferation and colony formation ability,induced cell cycle arrest and apoptosis in a dose-dependent manner.Regarding the metastasis potential,Mv-3-gal suppressed the migration and invasion potential of Huh-7 cells by regulating MMPs expression.Taken together,the findings highlighted the anti-HCC potent of Mv-3-gal,which was associated with the inhibition of Akt/PTEN,MAPK and MMP pathways.