AIM: To develop and validate a liquid chromatography-tandem mass spectrometric method (LC-MS/MS) for quantifying trimetazidine in human plasma. METHODS: Sample preparation was based on extracted using acetonitrile only. Chromatography was performed on a C18 analytical column and the retention times were 1.9 and 2.6 min for trimetazidine and cetirizine (internal standard), respectively. The ionization was optimized using ESI (+) and enhanced selectivity was achieved using tandem mass spectrometric analysis via two MRM functions, m/z:267→m/z:181 and m/z:389→m/z:201 for trimetazidine and cetirizine. RESULTS: The calibration curve ranged from 0.77 to 198 ng/mL. The inter-day precision and accuracy and the relative standard deviation (RSD) were<15%. The analyte was shown to be stable over the timescale of the whole procedure. The robustness of the method was demonstrated by the good reproducibility of the results obtained during the analysis of clinical samples. CONCLUSION: The experiment demonstrates an accurate, stable and reproducible LC-MS/MS method for quantifying trimetazidine in human plasma.
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