Partial sequence(314 bp) of ASPV was cloned and used as a probe labelled with digoxigenin-11dUTP.The total RNA extracted from samples with Apple stem pitting virus and a series of dilutions of plasmid with ASPV-cDNA were detected by dot blot hybridization.The results showed that the probe was sensitive and specific.The probe couldn’t hybridize with total RNA of Apple stem grooving virus,Apple mosaic virus and Apple chlorotic leaf spot virus samples as well as negative control,only hybridized with that extracted from dormant shoot infected with ASPV.The sensitivity for detection of plasmid contained ASPV-cDNA was 1.64 μg.
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