Phenylalanine ammonia lyase (PAL) has great values in industrial and potential medical applications, especially in the production of L phenylalanine from trans cinnamic acid. Strain breeding is important for the development and applications of this bioprocess. In this article, mutiple ways for breeding of microbial strains containing PAL were reviewed in details, including direction screening, enrichment culture technique for isolating strains from nature sources, phsical and chemical mutagenesis for strain improvement, PAL molecular clone and genetic engineering, etc. The significance of protoplast techniques for breeding of Rhodotorula strains containing PAL was analysed and discussed.
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