Pigs are suitable experimental animals for many surgical techniques[1-4]. Shumianning Ⅱ is compound anesthetic which cantain ketamine,xylazine and midazolam and can be only used for swine anesthesia. The purpose of this study was to evaluate the effects of Shumianning Ⅱ administered by continuous rate infusion using microinjection pump[5]on anesthetic quality,cardiopulmonary function and biochemistrical values in minipigs.The anesthestic quality of shumianning Ⅱ was investigated in 6 Bama minipigs,aged 8. 7 ± 0. 2 mon,mean weight 23. 7 ± 5. 0 kg. Minipigs were induced with shumianning Ⅱ 0. 03 mL/kg from the ear vein,then received with constant rate infusions of shumianning Ⅱ 0. 03 mL/kg·h for 1h by microinjection pump. Heart rate (HR),respiratory rate (RR),rectal temperature (RT),non-invasive arterial blood pressure (NABP),pulse oxygen saturation (SpO2) and end-tidal pressure of carbon dioxide (P ET CO2) were measured and the effectiveness of analgesia,sedation and muscle relaxation were observed every 5 min; the biochemical parameters of ALP,ALT,AST,CREA,UREA,GLU and TP were measured before and 1 h,24 h,48 h,72 h,168 h after the administration of Shumianning Ⅱ; 5 mL blood sample from the precaval vein was collected at 1 min,3 min,5 min,10 min,25 min,40 min,45 min,60 min,70 min,80min,90 min and 100 min,respectively,then the plasma concentrations of ketamine,xylazine and midazolam were measured with high-performance liquid chromatography.The results showed that the status of HR,RR,RT,NABP,SpO2and P ET CO2were significantly different from the base value at some time points (P0. 05),but all parameters were in the normal physiologic range. Levels of SpO2 at1 min and 5 min (P0. 05) were significantly lower than the normal physiologic range. Cardiovascular and blood chemistry effects was small,only the level of GLU was higher than the base value (P0. 05),while others were all not significantly different.In the period of intravenous infusion of Shumianning Ⅱ,the plasma concentrations of ketamine,midazolam and xylazine were downtrend in first 3 ~ 10 min,and began to raise at 14 min,the value reach the top at 20 ~ 55 min,the plasma concentration can be stable for about 1 h.Induction anesthesia in minipigs was quick,the stage of surgical anesthesia was long and smooth,recovery was smooth and quick. There was no pain reaction (eg. emesis,apnea,salivation) on every stage in the experiments. Using microinjection pump can be inexpensive,easier to perform. shumianningⅡmeets clinical operation requirement.
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