CYP3A4酶对土茯苓黄酮类成分代谢的影响

摘要

以土茯苓6种黄酮类成分为底物,研究了不同孵育时间、不同CYP3A4酶浓度对6种成分代谢的影响。通过土茯苓6种黄酮类成分的酶促动力学,得到米氏常数(Km)、最大反应速度(Vmax)和代谢清除率(Clint)。结果表明,以表儿茶素、新落新妇苷、落新妇苷、异落新妇苷、黄杞苷、白藜芦醇为底物时,Km分别为1.39,1.38,2.37,1.59,0.79,2.01μmol/L;Vmax分别为0.02,0.01,0.02,0.02,0.01,0.02μmol/(L·min);Clint分别为(1.14,0.96,0.90,0.99,1.42,0.97)×10^-2min^-1。