从培养基成分着手，研究了影响棉花离体根尖培养的各种因素，以建立棉花离体根尖培养体系。研究结果表明，棉花根尖培养的培养基为：MS大量元素+1／2 MS微量元素+100mg·L。肌醇+1．0mg·L。烟酸+10．0mg·L。盐酸硫胺素+L 0mg·L。盐酸吡哆辛+20mg·L。蔗糖+0．125mg·L。吲哚丁酸，用0．8％的琼脂为支撑物，调节pH值为6．4。培养温度为(28±2)℃，每天光照14 h，光照强度为2000 Lx。用此方法进行棉花根尖培养，离体根系可在培养基中生长达2个月而不影响其生活力。该棉花根尖培养体系适用于陆地棉和海岛棉，但基因型之间培养效果存在一定差异。%Conditions of cotton root tip culture invitro were studied in this paper. The recom-mended medium for cotton root tip culture invitro was macronutrients and iron salt of MS ba-sic medium, 1/2 micronutruents of MS basicmedium, vitamins and organic materials of B6basic medium containing 100mg · L-1 of inositol,1.0 mg· L1 of nicotonic acid, 10.0 mg· L-1 ofvitamin B1, 1.0 mg· L4 of vitamin B6, 20 mg ·L-1 of saccharose as the carbon resource andrn0. 125 mg · L-t of IBA, using 0. 8% of agar asthe solid material and adjusting pH to 6.4. Thecotton root tips were cultured at a photoperiodof 14 hours daily with a light intensity of 2000Lx and temperature of (28±2)C . The excitedcotton roots could grow very well in the mediumfor more than two months with this culturemethod. This culture system was suitable forthe materials of both G. hirsutum and G. bar-badense, though some differences were presentamong the genotypes.
展开▼
