利用RAPD对抗卷叶病品系CNH 123、CNH 1012和感病品系CNH 1020、CNH 120分别建立了抗、感病多态带,用80个引物进行PCR扩增.引物OPC 02在抗病品系CNH 123和CNH 1012中得到1700 bp的特征片段,建立了10个抗病及感病DNA库并进行混合分组分析,在F2群体用OPC 02重现了上述特征片段,并将该片段改造为SCAR标记,设计引物对为5'-GT-GAGGCGTCAGAGGGAT-3'(正向)and 5'-GTTGCCGTGCACTAGGCT-3'(反向).利用Mapmaker软件得到10个F2分离群体的RAPD分离图谱.%Random Amplified Polymorphic DNA(RAPD) analysis was carried in germplasm linesCNH 123 (Resistant to Cotton Leaf Curl Virus,RCLCuV), CNH 1012 (RCLCuV), CNH 1020(Susceptible to Cotton Leaf Curl Virus, SCLCuV)and CNH 120 (SCLCuV) to establish polymorphismamong the cotton leaf curl virus(CLCuV) resistantand susceptible genotypes. These lines were charac-terized using 80 decamer primers by amplification ina polymerase chain reaction. The primer OPC 02amplified a unique polymorphic fragment in the leafcurl virus resistant lines CNH 123 and CNH 1012designated as OPC 02 (1700 bp). Ten resistant andsusceptible F2 DNA were pooled for bulk segregantanalysis and amplified with the same primer OPC02, which also produced the 1700 bp fragment andconfirmed it repeatedly. This fragment has beenconverted into SCAR marker and the primer pair de-signed was 5'-GTGAGGCGTCAGAGGGAT-3'(forward) and 5'- GTTGCCGTGCACTAGGCT-3'(reverse). The F2 segregating RAPD loci weremapped using Mapmaker programme into ten groups.
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