Identification of Differentially Expressed Genes Associated with Cotton Fiber Development in a Chromosomal Substitution Line (CS-B22sh)

摘要

One of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development.Availability of chromosome arm substitution line CSB22sh (chromosome 22 short arm substitution from 3-79,Gossypium barbadense,into a TM-1,Gossypium hirsutum,background) provides a novel opportunity to study fiber-associated genes because previous studies revealed that this line was associated with some superior fiber quality traits compared to TM-1.We used an integrated approach of suppression subtractive hybridization (SSH),microarray,and real-time reverse treanscription-polymerase chain reaction(RT-PCR) technologies to identify the potential genes associated a SSH cDNA library with Chromosome substitution line CS-B22sh as the tester and TM-1 as the driver.