We conducted a tritrophic bioassay to evaluate the potential impact of the Cry1Ah protein on growth and the activities of development enzymes in Propylea japonica using cotton bollworm (Helicoverpa annigera) as prey.There were no significant differences in the survival rate,larval weight,larval development time,and pupation rate among P.japonica fed with cotton bollworm larvae reared an artificial diet containing Cry1Ah protein at concentrations of 0 (control),1.0,and 10.0 μg· g-1.The activities of some enzymes differed among the P.japonica fed with the different diets,but this did not affect the growth and development of the larvae.P.japonica fed with aphids showed significantly higher survival rate,larval weight,and pupation rate,and significantly shorter larval development time,compared with those of P.japonica fed with cotton bollworm.These results showed that different diets had a far greater effect on P.japonica than did the different concentrations of the CrylAh protein.%使用生物测定方法评价了Cry1Ah蛋白和食料对龟纹瓢虫幼虫的影响.研究结果表明,用1.0和10.0μg·g1的Cry1Ah蛋白处理下的棉铃虫饲喂龟纹瓢虫后,其存活率、幼虫虫体质量、幼虫发育时间和化蛹率均没有影响,但龟纹瓢虫体内某些酶活力有所改变,这种改变并未显著影响幼虫的生长发育.用蚜虫饲喂的龟纹瓢虫存活率、虫体质量和化蛹率显著高于棉铃虫处理,差异达显著水平；幼虫发育时间前者显著短于后者.研究结果表明食料的不同对龟纹瓢虫的影响远远大于Cry1Ah蛋白对其的影响.
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