以TM-1的染色体片段代换系CSB22sh和TM-1杂交,构建了包含104个家系的重组自交系(RILs)群体.利用74对具有多态性位点的引物进行检测,构建了包含61个多态性位点,长度为76.93 cM的遗传图谱,平均标记间距离1.26 cM.利用此遗传图谱结合重组自交系群体4个环境下的5个纤维品质性状进行QTL定位,共定位出12个QTLs,解释性状表型变异的2.45％～21.11％;在1,2,3,4个环境中同时检测到的QTLs分别有9,1,1,1个.而利用4个环境平均值进行联合分析定位出个4个QTLs,纤维长度和纤维整齐度的QTLs均为2个,解释性状表型变异的14.37％～19.97％,并且纤维长度和整齐度的QTL在相同的位置.多环境下检测到的QTL可能对标记辅助选择有实际应用价值.%A population of 104 recombinant inbred lines (RILs) was developed by single seed descended from the cross of upland cotton (Gossypium hirsutum L.) TM-1 and its substitution line, CSB22sh. Agenetic linkage map consisting of 61 loci and covering 76.93 cM was constructed using SSR markers, with the average distance of 1.26 cM between two markers. Composite interval mapping was used to identify the quantitative trait loci (QTL) in RILs, 12 QTLs for fiber quality were independently detected in four environments, whereas 4 QTLs were found in joint analysis. These QTLs explained 2.45%～21.11% of the phenotypic variance. There were 9, 1, 1 and 1 QTLs simultaneously significant in one, two, three and four environments, respectively. There were two fiber length and two fiber uniformity QTLs in joint analysis, which explained 14.37% ～ 19.97% of the phenotypic variance and in same position respectively. Notably, a stable fiber length QTL qFl-22-2 was detected both in joint analysis and in four environments of separate analysis, which might be of special value for marker-assisted selection.
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