目的：探讨高危型HPV/TCT检测在宫颈CINⅠ随访中的应用。方法将2010年1月-2013年1月妇科门诊及住院收治的188例宫颈上皮内瘤变患者进行TCT检测，运用第二代杂交捕获实验的方式进行免疫学和化学发光技术让基因信号放大的微孔板对标本中的病毒进行HR-HPV的检测，颈管刷采集宫颈脱落细胞进行液基薄层细胞涂片，TBS分级系统进行细胞学检测。结果188例患者中炎症占78.19%，ASC-US及以上病变细胞学阳性率21.88%，CINⅠ病变阳性率17.55%，二者相比，差异没有统计学意义（χ2=0.05，P＞0.05）；HR-HPV阳性率17.02%，包括慢性炎症阳性率为7.43%（11/148），CINⅠ及以上阳性率为52.5%，二者相比差异有统计学意义（χ2=45.64，P＜0.05）。结论高危型HPV/TCT检测能够作为ASCUS患者的分流手段，降低漏诊率。%Objective To research the application of high risk type HPV/TCT detection in the follow-up of cervical intraepithelial neoplasiasⅠ. Methods 188 outpatient and hospitalized patients of department of gynaecology with cervical intraepithelial neoplasia, from January 2010 to January 2010, were treated with TCT detection. Detecting high-risk human papillomavirus by using the second generation of hybrid capture experiment immunological and chemiluminescence technology for gene with the method of signal amplification of microporous surface specimens. Cervical exfoliated cells were collected with neck tube brush for liquid thin layer cell smear. TBS classification system was used for cytological examination. Results The inflammation rate in 188 patients was 78.19%, ASC-US and above lesions cytology positive rate was 21.88%,and CINⅠlesions positive rate was 17.55%. And the difference was no statistical significance (χ2=0.05, P>0.05).The high-risk human papillomavirus rate was 17.02%. And the positive rate chronic inflammation was 7.43% (11/148), CINⅠ and above positive rate was 52.5%. And the difference was statistical significance(χ2=45.64,P<0.05). Conclusion HR-HPV/TCT detection can be used as a shunt means, decrease the rate of missed diagnosis of ASCUS patients.
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