AKT1和AKT2蛋白在胶质母细胞瘤中的表达及对患者预后的影响

摘要

目的 探讨AKT1和AKT2蛋白在胶质母细胞瘤中的表达及对患者预后的影响.方法 选择我院收治并确诊的胶质母细胞瘤93例(观察组)和同期行脑血管畸形手术切除的正常脑组织20例(对照组),运用免疫组织化学染色法检测AKT1和AKT2蛋白的表达情况,采用Kaplan-Meier生存曲线对患者总生存期进行分析,应用Cox比例风险回归对影响胶质母细胞瘤患者预后的变量进行多因素分析.结果 观察组AKT1与AKT2蛋白表达阳性率分别为74.19％ (69/93)、67.74％ (63/93),高于对照组的5％(1/20)、15％(3/20),差异有统计学意义(P＜0.05).Kaplan-Meier生存分析显示,AKT1和AKT2蛋白表达阳性者总生存期低于蛋白表达阴性者,差异有统计学意义(P＜0.05).Cox比例风险回归分析显示,手术切除程度、辅助治疗、AKT1和AKT2蛋白的表达为胶质母细胞瘤预后的独立危险因素(P＜0.05).结论 AKT1和AKT2蛋白的表达与胶质母细胞瘤患者预后密切相关,可作为判断预后的指标之一.%Objective To investigate the expression and prognostic value of AKT1 and AKT2 in patients with glioblastoma (GBM).Methods AKT1 and AKT2 expression was assessed by immunohistochemistry in 93 cases of GBM (tumor group) and 20 normal brain tissues (control group) which were obtained intraoperatively in patients with cerebral vascular malformation.Overall survival (OS) was evaluated graphically with Kaplan-Meier curves.The multivariate analysis for OS was performed using Cox regression analysis.Results The positive expressions of AKT1 and AKT2 in tumor group were 74.19％ (69/93) and 67.74％ (63/93) respectively,which were significantly higher than those in control group (5％,1/20;15％,3/20).Kaplan Meier method showed patients with positive AKT1 or AKT2 expression had significantly shorter OS relative to those with negative expression,indicating significant differences (P ＜ 0.05).The multivariate Cox regression analysis revealed that extent of surgical resection,adjuvant treatment,and AKT1 and AKT2 expression were independent risk factors for OS (P ＜ 0.05) in patients with GBM.Conclusion The expression of AKT1 and AKT2 may be associated with prognosis of GBM,and could be considered as significant prognostic indicators.