以污水处理厂氧化沟污泥为泥种,采用进水低碳高磷、两阶段的运行方式进行反硝化聚磷污泥的培养,约100 d成功驯化培养出反硝化聚磷污泥.第1阶段以厌氧/好氧的运行方式驯化好氧聚磷污泥,运行约40 d,最大释磷量、最大聚磷量和最大除磷量分别可达到77.2、89.4、25.0 mg/L,表现出较强的聚磷能力；第2阶段采用厌氧/缺氧/好氧的运行方式驯化反硝化聚磷污泥,运行60 d,缺氧聚磷量占总聚磷量的百分比呈上升趋势.硝化污泥经过100 d的驯化可去除约50 mg/L的氨氮,硝化率基本稳定在98.5％以上.硝化速率本符合零级动力学方程,比硝化速率常数为0.0024h-1；好氧聚磷速率和缺氧聚磷速率基本符合一级动力学方程,速率常数分别是0.377、0.740 g/(L·h-1).利用驯化培养成功的反硝化聚磷污泥和硝化污泥进行了A2N-SBR试验,结果表明:在进水COD、氨氮和磷分别为188.0、54.8、7.25 mg/L时,去除率分别为93.5％、76.7％和94.1％,驯化培养的双污泥具有良好的脱氮除磷效果.%In the experiment,the denitrifying phosphorus removing bacteria (DPB) was cultivated by high phosphorus and low carbon source influent.It was cultivated successfully by the 2-stage mode for about 100 d.The first stage was about 40 d,which was with the mode of anaerobic/aerobic to enrich the phosphorus accumulating organisms (PAOs).The maximum amounts of the phosphorus release,phosphorus uptake and phosphorus removal were 77.2 mg/L,89.4 mg/L and 25.0 mg/L respectively.The second stage was 60 d,which was with the mode of anaerobic/anoxic/aerobic.In this stage,the ratio of denitrifying phosphorus uptake amount to the total phosphorus uptake amount had an ascending tendency.The cultivation of nitrifying activated sludge was about 100 d,with the 50 mg/L ammonia removal amount and more than 08.5% ammonia removal efficiency.Nitrification rate fitted zero order kinetic equation,and the specific nitrification rate constant was 0.002 4 h-1.Phosphorus uptake rates fitted first order absorption kinetic equation.The uptake rate constants under aerobic and anoxic conditions were 0.377 and 0.740 L/(g · h-1).The cultivated DPB sludge and nitrifying activated sludge were used in an anaerobic/ anoxicitrification-sequencing batch reactor (A2N - SBR) process to remove nitrogen and phosphorus.When the concentrations of influent COD,ammonia and phosphorus were 188.0 mg/L,54.8 mg/L and 7.25 mg/L,the removal efficiencies of COD,ammonia and phosphorus were 93,5%,76.7% and 94.1%rnrespectively.
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