NF-κB1基因启动子区插入/缺失(ATTG)多态性与膀胱移行细胞癌易感性的关系

摘要

目的 了解核因子-κB1(NF-κB1)基因启动子区插入/缺失(ATTG)多态性与膀胱癌易感性的关系.方法 采用聚合酶链反应(PCR)及聚丙烯酰胺凝胶电泳检测207例膀胱移行细胞癌患者(膀胱癌组)和228例健康者(对照组)的NF-κB1基因型,并进行对比分析.结果 膀胱癌组的ATTG2等位基因频率(65.2%)明显高于对照组(56.1%,P＝0.006,OR=1.465),膀胱癌组ATTG2等位基因频率是ATTG1等位基因频率的近两倍.结论 NF-κB1基因启动子区的多态性与膀胱癌移行细胞的易感性有关.%Objective To understand the association of insertion/deletion(ATTG) polymorphism in the promoter region of NF κB1 gene with susceptibility to transitional cell carcinoma of bladder. Methods Polymerase chain reaction and polyacrylamide gel electrophoresis were employed to detect NF-κB1 genotypes in 207 patients with transitional cell carcinoma of bladder(bladder carcinoma group) and 228 healthy people(control group),and these NF-κB1 genotypes were comparatively analyzed. Results The frequency of ATTG2 allele in bladder carcinoma group(65.2 ％ )was significantly higher than that of ATTG2 in control group(56.1 ％,P=0.006 ,OR=1.465). In the bladder carcinoma group,the frequency of ATTG2 allele was nearly twice higher than that of AT TG1 allele. Conclusion Polymorphism in the promoter region of NF-κB1 gene is associated with susceptibility to transitional cell carcinoma of bladder.