组织型纤溶酶原激活物在进展期大肠癌组织中的表达与微血管密度的相关性研究

摘要

目的 探讨组织型纤溶酶原激活物(t-PA)在进展期大肠癌组织中的表达与微血管密度(MVD)的关系.方法 选择60例进展期大肠癌癌组织为研究组,其中20例癌旁正常大肠组织为对照组.用发色底物法检测外缘癌组织浸液的t-PA浓度,用免疫组化SP法检测大肠癌外缘组织的MVD.结果 进展期大肠癌外缘癌组织浸液t-PA浓度低于正常大肠组织(P<0.01),外缘癌组织MVD高于正常组织(P<0.01),外缘癌组织t-PA表达与MVD呈负相关(r=-0.570 8,P<0.01).结论 进展期大肠癌可能通过调低t-PA浓度造成局部高凝状态,从而促进微血管的生成.%Objective To investigate the correlation between tissue type plasminogen activator (t PA) in advanced colorectal cancer tissue and microvessel density(MVD). Methods We selected 60 cases with advanced colorectal cancer tissue as the research group and 20 cases with normal para cancer tissues as the control group. The t PA concentration of cancer tissue extracts was detec ted by Chromogenic substrate method; the MVD was detected by immunohistochemical SP method. Results Extracts of the outer edge cancer tissue had a lower t PA concentration and a higher MVD than normal colon tissue(P<0. 01). T PA expression in cane er tissues had a negative correlation with MVD(r= - 0. 570 8,P<0. 01). Conclusion Advanced colorectal cancer could cause hy percoagulability by lowering t PA concentration,thus promoting the formation of capillaries.